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Evaluation of tooth preparation using Er: YAG laser in comparison with other preparation methods
Bučková, Michaela ; Dostálová, Taťjana (advisor) ; Racek, Jaroslav (referee) ; Mazánek, Jiří (referee) ; Smrčka, Pavel (referee)
Er: YAG laser is a new method of preparation that promises gentler and more accurate dental treatment. Its specific characteristics is large absorption of the beam in the water and a small absorption in the tissue without the thermal effect. Therefore the treatment is safer and effectiver. The research has the aim in its theoretical - cognitive part to assess its advantages in comparison with treaditional treatment method - drill. As an essential element will be evaluation of caries lesion in vitro and in vivo. The size of the cavity will be judged on, the shape of developing a smear layer and retention surface in relation to the filling and the . The extracted teeth will be scanned in electronic microscope to assess the formation of contact and non-contact preparation, focusing on the shape and size of the prepared lesion in relation to modern filling materials - low viscosity composites. Extensive research supported by the Ministry of Health (IGA : 9991-4 and 13351-4) that focuses on the the use of various therapeutic methods in the treatment of special needs patients. Dental treatment of a special needs patients is expensive and time-consuming than conventional dental treatment. Therefore, clinical application of theoretical cognitive part will create a electronical support system for the...
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Taxidermal Mounts of the Fish Body
Chlad, Martin ; Řezníček, Jan (advisor) ; Hanel, Lubomír (referee)
The thesis presents methodology of creating whole-fish dermoplastic models. It is primarily intended for teachers of natural history and biology. The results are based on mapping out both modern and traditional methods of fish taxidermy. By empirical verification and introduction of new methods, a comprehensive manual of fish taxidermy was created. It can serve as a useful didactic tool. Methodology also describes the issue of obtaining and preserving fish specimen. Profound knowledge of fish anatomy is a necessary prerequisite for specimen taxidermy, thus methodology also includes anatomical overview and guidelines for fish anatomical dissection. The output of the practical part of the thesis are dermoplastic fish models which became part of the specimen collection at the Department of Biology and Environmental Studies at the Faculty of Pedagogics at Charles University, where they are meant to be used for educational purposes. Methodological part of the thesis evaluates the experience gained during the process of model creation. Certain recommendations regarding suitability of particular fish species for taxidermy, material and tools required, choice of method and its advantages and disadvantages, were made. The thesis discusses several specific technologies, such as producing eyes for the...
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Parasites And Their Preparation For School Utilization
Chlumová, Kateřina ; Říhová, Dagmar (advisor) ; Hanel, Lubomír (referee)
This diploma thesis deals with common parasites of game animals, animals and human occurring in the territory of the Czech Republic. It focuses on their fixation and general preparation of such specimens for usage in teaching of Science. The first part is devoted to the phenomenon of parasitism, and the history and definition of this concept. Systematic hierarchy of various unrelated parasitic groups is clarified. The second part provides the list of parasites, from the evolutionary original to the highly derived groups. We give an overview of their morphology and anatomy with the emphasis on their life cycle. The detailed knowledge of life cycle is essential for obtaining specimens. In each group, we indicate methods of collection and fixation in detail. The co called smartmicroscope represent the main point of the third part of proposed diploma thesis. Text of the thesis is supplemented with pictures taken by smart microscope, as well as by common microscope. KEY WORDS parasite, parasitism, mikropredatore, vector, fixation, preparation, smart mikroscope
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Chemo-enzymatic synthesis of antiviral prodrugs
Tupec, Michal ; Stiborová, Marie (advisor) ; Indra, Radek (referee)
Lipases have been widely applied in the manufacture of food products and in some areas of the industry, nowadays they are used in synthetic organic chemistry catalyzing the hydrolytic/esterification reactions under very mild conditions in the field of protecting groups or enantiomer resolution. In this study, the commercial lipase from bacterium Pseudomonas fluorescens was immobilized using the sol-gel process into organosilicate materials with propyl, octyl or phenyl substituents. The highest hydrolytic activity was found in the enzyme on the octyl-derived carrier. The immobilized enzymes differ in their hydrolytic activities on 4-nitrophenyl esters of various lengths. Subsequent experiments revealed quite good pH stability of the enzymes in a buffer (incubations in pH 3 through pH 11), as well as good temperature stability in isooctane (incubations at up to 100 řC). The majority of organic solvents seem to have no substantial effect on the lipase activity. The biocatalytic properties were studied on a model compound from the group of the acyclic nucleoside analogues - 9-(2',3'-dihydroxypropyl)adenine (DHPA). It was found for example that the best acyl donors are vinyl esters, that the lipase shows a preference towards longer vinyl esters, that the reaction proceeds faster in non-polar solvents or that it...
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Taxidermal Mounts of the Fish Body
Chlad, Martin ; Řezníček, Jan (advisor) ; Hanel, Lubomír (referee)
The thesis presents methodology of creating whole-fish dermoplastic models. It is primarily intended for teachers of natural history and biology. The results are based on mapping out both modern and traditional methods of fish taxidermy. By empirical verification and introduction of new methods, a comprehensive manual of fish taxidermy was created. It can serve as a useful didactic tool. Methodology also describes the issue of obtaining and preserving fish specimen. Profound knowledge of fish anatomy is a necessary prerequisite for specimen taxidermy, thus methodology also includes anatomical overview and guidelines for fish anatomical dissection. The output of the practical part of the thesis are dermoplastic fish models which became part of the specimen collection at the Department of Biology and Environmental Studies at the Faculty of Pedagogics at Charles University, where they are meant to be used for educational purposes. Methodological part of the thesis evaluates the experience gained during the process of model creation. Certain recommendations regarding suitability of particular fish species for taxidermy, material and tools required, choice of method and its advantages and disadvantages, were made. The thesis discusses several specific technologies, such as producing eyes for the...
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Parasites And Their Preparation For School Utilization
Chlumová, Kateřina ; Říhová, Dagmar (advisor) ; Hanel, Lubomír (referee)
This diploma thesis deals with common parasites of game animals, animals and human occurring in the territory of the Czech Republic. It focuses on their fixation and general preparation of such specimens for usage in teaching of Science. The first part is devoted to the phenomenon of parasitism, and the history and definition of this concept. Systematic hierarchy of various unrelated parasitic groups is clarified. The second part provides the list of parasites, from the evolutionary original to the highly derived groups. We give an overview of their morphology and anatomy with the emphasis on their life cycle. The detailed knowledge of life cycle is essential for obtaining specimens. In each group, we indicate methods of collection and fixation in detail. The co called smartmicroscope represent the main point of the third part of proposed diploma thesis. Text of the thesis is supplemented with pictures taken by smart microscope, as well as by common microscope. KEY WORDS parasite, parasitism, mikropredatore, vector, fixation, preparation, smart mikroscope
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Taxidermal Mounts of the Reptiles, Small Mammals and Fishes
Kubátová, Claudie ; Řezníček, Jan (advisor) ; Poláchová, Tereza (referee)
1 ABSTRACT The thesis is conceived as detailed guide to create a dermoplastic models for teachers of natural history and biology. The first part focuses on the actual field of taxidermy and its methods. It includes some possible methods leading to the making of dermoplastic models of mammals, reptiles and fishes. The second part describes the created models of mammals, reptiles and fishes. This section also contains the characteristics of individual species and recent research from specialized foreign literature.
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Taxidermy and skeletal mounts in the vertebrates
Zámečníková, Nikola ; Řezníček, Jan (advisor) ; Tuzová, Lucie (referee)
This final thesis represents a complete guide to making didactic aids which can be used in teaching Biology in primary and secondary schools. It completes my bachelor thesis on the topic of the Taxidermy of selected species of birds with detailed photographs of individual steps made throughout the creation process of a dermoplastic mount. It also compares the creation of bird mounts to those of mammal mounts, and it deals with making skeletons of vertebrates. This final thesis acquaints the reader with the taxidermic species, and includes research of foreign literature focussing on interesting facts about mounts. The final output of this thesis is a series of mounts and assembled skeletons of different species of vertebrates, which can all be used as didactic aids at the Department of Biology and Environmental Studies, Faculty of Education, Charles University. KEY WORDS taxidermy, skeletons, vertebrates, didactic aids, dermoplastic mount
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Taxidermy of Small Mammals and Passerines
Pulková, Nikola ; Řezníček, Jan (advisor) ; Ouřadová, Petra (referee)
01 Abstract Diploma thesis deals with taxidermy techniques of birds and small mammals leading to the production of own taxidermal mounts, which can be afterwards used as suitable didactical tools in the education of biology. Thus the thesis is presenting a comprehensive guide for creation of taxidermal mounts. At the same time, all the the stated procedures are applied at couple of species used for taxidermical purposes. Apart from the techniques itself which are described in methodological part, the thesis is enriched with the description of individual groups and therefore, creates theoretical introduction about the species. The thesis also depicts the production of all described taxidermal mounts. Results of this thesis are in total 8 taxidermal mounts (3 birds and 5 mammals), additionally also osteological material in the form of skeleton of Apodemus sylvaticus and skulls of 3 described small mammals. All the stated taxidermal mounts will serve as didactical tools for needs of Department of Biology and Environmental Studies at Faculty of Education in Prague. Key words: dermoplastic models, taxidermal mounts, osteological material, taxidermy, birds, mammals
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